Vitamin D levels should be routinely examined in patients who are obese, have mal-absorption syndrome, have softening of the bones (osteomalacia), or are receiving therapy for tuberculosis, according to Dr. Prashant Nag, Clinical Head, Tata 1mg Labs. Regular full-body exams, which should be performed at least once a year and every six months, can also evaluate vitamin D levels. The most susceptible groups to a vitamin D deficiency are young children and infants under the age of five, pregnant and nursing women, teenagers and young adults, those over 65, and those who get little sun exposure.