Technique based on laser speckles can identify hearts suitable and safe for transplantation

France: The newly developed imaging technique enables precise visualization of blood circulation. In the future, this technique can potentially identify myocardial perfusion abnormalities that indicate underlying heart conditions, such as coronary artery disease.

According to Amy Oldenburg, Professor at the University of North Carolina at Chapel Hill and JBO Associate Editor, “The noncontact technique, based on laser speckle, provides imaging of blood circulation within donor's hearts and has important implications for identifying hearts that are safe and suitable for transplantation."

Here’s hoping these findings can make heart transplantation surgeries safer and more successful for patients needing this life-saving procedure.

In most cases, graft failure after heart transplantation is attributable to abnormalities like severe coronary artery disease. As donors with extended criteria like advanced age and pre-existing heart conditions become eligible for heart transplantation, careful screening for congenital abnormalities has become crucial. Invasive coronary angiography is an essential screening tool to detect coronary artery disease (CAD), characterized by cholesterol deposits in the heart's arteries. However, logistical challenges limit utility, so it’s used for fewer than a third of donors who are at risk of developing CAD.

A new heart preservation procedure called “ex-situ heart perfusion” (ESHP) has been developed to overcome this limitation. By supplying oxygenated nutrients to the heart via blood vessels, ESHP allows doctors to monitor the heart's performance and screen it for any defects outside the body. However, coronary angiography conducted during ESHP is known to damage the heart and result in primary graft failure. Therefore, alternative coronary imaging tools are required to identify abnormal blood flows in donor's hearts and determine their eligibility for transplantation.

As reported in the Journal of Biomedical Optics (JBO), a team of researchers led by Professor Elise Colin from Paris Saclay University, France, introduced a safe and noninvasive optical technique that allows imaging of coronary blood circulation in donor hearts during ESHP. The method utilizes a recently developed speckle imaging technique, Laser Speckle Orthogonal Contrast Imaging (LSOCI), developed to detect the multiple scattering of moving red blood cells.

In this study, the researchers improved the imaging capability of LSOCI to observe small blood vessels in the heart. The proposed method thus analyzes blood flow in the heart using a specific polarimetric filtering process that allows suppression of surface scattering. Thus, the time-varying speckle patterns used for the imaging process are mainly generated by multiple scattering on moving red blood cells inside peripheral vessels.

“The optical technique allows high-resolution imaging of the entire peripheral vasculature of the heart in real-time,” says Colin.

To test this method, the researchers developed a clinical model to study coronary circulation on donor hearts before transplantation. They then used a laser and a camera mounted on an articulated arm fixed above the perfusion module which contained the donor heart-to generate and analyze rapidly varying speckle patterns.

To overcome the challenges in tracking vasculature due to the beating of the heart, the researchers further optimized the technique with a method called Multi-Period-Enhanced Signal-to-Noise Ratio (MPE-SNR). They took a series of images over time to build a set of frames that depicted the vasculature at similar heart positions. Each image in this sequence was then optimized using the other images to reduce noise and enhance details. The optimized image represented the vasculature at a different time point. The researchers used a sequence of such images to visualize vasculatures as small as 100 microns in a few seconds.

Reference:

Aurélien Plyer, Elise Colin, Xavier Orlik, Ali Akamkam, Julien Guihaire, Journal of Biomedical Optics, (April 2023). https://doi.org/10.1117/1.JBO.28.4.046007.