From pulp to cementum: 3D visualization of soft and hard dental tissues using different ex vivo nano-CT contrast-enhancement techniques
In a new study, researchers found that decalcification followed by Lugol's iodine treatment significantly improved soft tissue contrast, particularly for pulp visualisation. In contrast, PTA without decalcification provided better contrast for dentine and allowed clear visualisation of attached soft tissues like the periodontal ligament and predentine. These results help guide the selection of imaging protocols tailored to specific dental tissue analyses.
A study was done to determine the effect of two contrast-enhancement strategies in nano-computed tomography (nano-CT) imaging on the contrast-to-noise ratio (CNR) of various dental tissues, including pulp, dentine and cementum, with the goal of enhancing the visibility of dental soft tissues to a level not yet reported in laboratory nano-CT imaging. Ten sound human third molars underwent decalcification and subsequent treatment with Lugol's iodine (n = 5, Group 1) or phosphotungstic acid (PTA) treatment without prior decalcification (n = 5, Group 2) for contrast enhancement.
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