Study describes a non-invasive method for evaluating burn injuries and predicting wound healing

USA: A neural network model that uses terahertz time-domain spectroscopy (THz-TDS) data for the non-invasive evaluation of burn injuries has been described in an article published in the peer-reviewed journal Biomedical Optics Express.

The researchers combined the new approach with a handheld imaging device that they developed specifically for fast THz-TDS imaging of burn injuries.

THz-TDS uses short pulses of terahertz radiation to probe a sample. It is being examined for assessing burn injuries because physical changes caused by a burn will produce alterations in the skin’s terahertz reflectivity.

“It is important for healthcare professionals to accurately assess the depth of a burn to provide the most appropriate treatment,” said research team leader M. Hassan Arbab from Stony Brook University. “However, current methods of burn depth evaluation, which rely on visual and tactile examination, be unreliable, with accuracy rates hovering around 60-75%. Our new approach could potentially improve the accuracy of burn severity assessments and aid in treatment planning.”

The researchers report the results showing that their artificial neural network classification algorithm can accurately predict the ultimate healing outcome of in vivo burns in an animal study with 93% accuracy. Compared to previous machine learning approaches used by the researchers, the new method reduces the necessary training data by at least two orders of magnitude. This could make processing big data sets obtained over large clinical trials more practical.

“In 2018, approximately 416,000 patients were treated for burn injuries in emergency departments in the United States alone,” said Arbab. “Our research has the potential to significantly improve burn healing outcomes by guiding surgical treatment plans, which could have a major impact on reducing the length of hospital stays and number of surgical procedures for skin grafting while also improving rehabilitation after injury.”

Better burn assessment

Various technologies have been developed to improve burn assessment, but they haven’t been widely adopted in the clinic due to drawbacks such as long acquisition times, high costs and limited penetration depth and field of view. Although THz-TDS looks promising for burn assessment, early demonstrations were limited to point spectroscopy measurements, which don’t account for burn heterogeneity and spatial variations. THz spectroscopy setups also tend to be bulky and expensive and require cumbersome optical alignments, making them impractical for clinical use in real-world settings.

“To address these challenges, we developed the portable handheld spectral reflection (PHASR) scanner, a user-friendly device for fast hyperspectral imaging of in vivo burn injuries using THz-TDS,” said Arbab. “This handheld device uses a dual-fiber-femtosecond laser with a center wavelength of 1560 nm and terahertz photoconductive antennas in a telecentric imaging configuration for the rapid imaging of a 37 x 27 mm2 field of view in just a few seconds.”

Previously, the researchers used numerical methods to extract features from the THz-TDS images and machine learning techniques to estimate the severity grade of in vivo burn injuries using measurements from the PHASR scanner. However, this approach did not consider the physical dynamics and macroscopic changes of the dielectric permittivity of burned skin tissue. Dielectric permittivity describes how a material responds to an electric field.

To investigate the mechanisms that change the complex dielectric function of skin burns in terahertz frequencies, the researchers turned to the double Debye theory, which has been successfully used to explain the interaction of THz radiation with various types of biological tissue.

Predicting severity and healing

“We developed a neural network model utilizing the five parameters obtained from fitting the double Debye model to the dielectric permittivity of burn injuries,” said Arbab. “This physics-based approach allows for the extraction of biomedical diagnostic markers from broadband THz pulses, reducing the dimensionality of THz data for training the artificial intelligence models and improving the efficiency of machine learning algorithms.”

The researchers tested their method by using the PHASR scanner to obtain spectroscopic images of skin burns and measure the permittivity of the burns. After determining the Debye parameters, the researchers used this data to create a neural network model based on labeled biopsies. The model estimated the severity of the burns with an average accuracy rate of 84.5% and predicted the outcome of the wound healing process with an accuracy rate of 93%.

The researchers note that clinical testing of both the technique and the handheld imaging device is needed before this technique can be integrated into the existing workflow of clinical burn assessment.

Reference:

Mahmoud E. Khani, Zachery B. Harris, Omar B. Osman, Adam J. Singer, and M. Hassan Arbab, "Triage of in vivo burn injuries and prediction of wound healing outcome using neural networks and modeling of the terahertz permittivity based on the double Debye dielectric parameters," Biomed. Opt. Express 14, 918-931 (2023)