Found: A way to grow retinal nerve cells in lab

The process entails genetically modifying a line of human embryonic stem cells to become fluorescent upon their differentiation to retinal ganglion cells, and then using that cell line for development of new differentiation methods and characterisation of the resulting cells.

Using a genome editing tool called CRISPR-Cas9, the researchers inserted a fluorescent protein gene into the stem cells' DNA. This red fluorescent protein POU4F2 would be expressed only if another gene named BRN3B was also expressed. BRN3B is expressed by mature retinal ganglion cells, so once a cell differentiated into a retinal ganglion cell, it would appear red under a microscope.

Next, they used a technique called fluorescence-activated cell sorting to separate the newly differentiated retinal ganglion cells from a mixture of different cells into a highly purified cell population.

The cells showed biological and physical properties seen in retinal ganglion cells.

Researchers also found that adding a naturally occurring plant chemical called forskolin on the first day of the process helped improve the cells' efficiency of becoming retinal ganglion cells.