Zirconium oxynitrate etchant preserves hybrid layer when used with simplified bonding systems: Study
Recent research conducted at the Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Bologna, Italy has come to the conclusion that experimental zirconium oxynitrate etchant demonstrated promising results in hybrid-layer preservation over time when used with simplified bonding systems.
Edoardo Mancuso and associates carried out the study to investigate, by means of microtensile bond strength test (µTBS), nanoleakage expression analysis (NL), gelatin zymography and in situ zymography, the effects of an experimental metal salt-based zirconium oxynitrate etchant [ZrO(NO3)2,] – ZON with two simplified adhesives on long-term bond strength and endogenous enzymatic activities.
Middle/deep coronal dentin surfaces (N = 32) were conditioned either with a traditional 37% H3PO4 etchant (TE) or with ZON. Further, a single-component etch-and-rinse adhesive (EF) or a universal adhesive (AU) were applied and µTBS and NL tests were performed.
Additional freshly extracted teeth were processed for gelatin zymography and in situ zymography evaluation. The tests were performed at baseline and (T0) and after 1-year-aging (T12). Bond strength and in situ zymography results were analyzed using analysis of variance (ANOVA) (three-way and one-way, respectively), while Chi-squared test was used for the NL results. Statistical significance was preset at α = 0.05.
The results showed that-
a. All the investigated factors (adhesive system, dentin conditioner and aging) significantly influenced µTBS, with the AU and ZON performing better compared to EF and TE, respectively, and with lower bond strength values after aging (p < 0.05). b. Incremented silver nitrate deposits were observed at the adhesive interfaces after aging, especially for the TE groups (p < 0.05).
c. Further, the experimental groups treated with ZON had significantly lower levels of enzymatic activity compared to TE, as shown by gelatin and in situ zymography (p < 0.05).
Therefore, the authors concluded that "The experimental etchant demonstrated promising results in hybrid-layer preservation over time when used with simplified bonding systems, and could therefore be recommended in the clinical practice."