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New approach to preserving organs for later use

Written by savita thakur thakur Published On 2015-12-02T11:02:37+05:30  |  Updated On 2 Dec 2015 11:02 AM IST
New approach to preserving organs for later use
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New York: Researchers at Oregon State University (OSU) have discovered a new approach to preserving tissues and even organs for later use.

"This could be an important step toward the preservation of more complex tissues and structures," said Adam Higgins from OSU.

Ice-free cryopreservation has already found widespread use in simpler applications such as preserving semen, blood, embryos, plant seeds and some other biological applications.

But it is often constrained by the crystallisation that occurs when water freezes, which can damage or destroy tissues and cells, Higgins said.

To address this, researchers have used various types of cryoprotectants that help reduce cell damage during the freezing process - among them is ethylene glycol, literally the same compound often used in automobile radiators to prevent freezing.

A problem, Higgins said, is that many of these cryoprotectants are toxic, and can damage or kill the very cells they are trying to protect from the forces of extreme cold.

In the new OSU research, the engineers developed a mathematical model to simulate the freezing process in the presence of cryoprotectants, and identified a way to minimise the damage.

They found that if cells are initially exposed to a low concentration of cryoprotectant and time is allowed for the cells to swell, then the sample can be vitrified after rapidly adding a high concentration of cryoprotectants.

The end result is much less overall toxicity, Higgins said.

The research showed that healthy cell survival following vitrification rose from about 10 percent with a conventional approach to more than 80 percent with the new optimised procedure.
savita thakur thakur
savita thakur thakur
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