Cystic fibrosis drug helpful for pneumonia treatment, study finds

Germany: The researchers from Berlin have discovered a potential new therapy in the form of a cystic fibrosis drug that proved effective in their laboratory experiments. The discovery raised hope that it could treat pneumonia regardless of the causative pathogen. The findings from the study were published in the journal Science Translational Medicine.

Pathogens such as SARS-CoV-2 and pneumococcus can cause severe pneumonia. If the airways fill with fluid, the patient risks developing acute respiratory distress syndrome. The research team have discovered the molecular mechanisms that trigger fluid accumulation in the lungs.

Pneumonia is the most common cause of fluid buildup in the lungs. This condition, known as pulmonary edema, results in parts of the airspaces filling with fluid instead of air, preventing them from exchanging gases. Patients struggle to breathe, and their bodies can't get enough oxygen. The diagnosis is acute respiratory distress syndrome or ARDS. "Despite cutting-edge medical procedures, roughly 40 percent of patients with ARDS die in intensive care. The problem is that antibiotics, antivirals, and immune-modulating therapies rarely work well enough," says study leader Prof. Dr. Wolfgang Kuebler, Director of the Institute of Physiology at Charité.

"That's why we took a very different approach in our study. Instead of focusing on the pathogen, we focused on strengthening the barrier function of the blood vessels in the lungs." This makes sense, as they are the fluid source in pulmonary edema. The lung vessels become permeable, allowing fluid from the blood to flow into the surrounding tissue and thereby flood the airspaces.

But what causes this? What are the underlying molecular mechanisms? A Charité research team led by Prof. Kuebler set out to answer these questions. They performed experiments using cells, lung tissue, and isolated lungs. The study centered on the CFTR chloride channel, which scientists know is mainly found in the mucosal cells of our airways. There, it plays a major role in keeping our mucus thin so it can drain away easily. The researchers have now shown for the first time that cells in the lungs' blood vessels also have CFTR and that its presence is drastically reduced in pneumonia.

To find out what role CFTR plays in the pulmonary vessels and what is happening at the molecular level when the chloride channel is lost, the researchers blocked the channel with an inhibitor and dictated the number of chloride ions in the cells. They then used a special imaging technique known as immunofluorescence imaging: "We saw that inhibiting CFTR triggered a molecular cascade that ultimately causes the lung's blood vessels to begin leaking," says Dr. Lasti Erfinanda, who also works at the Institute of Physiology and is the study's lead author. "So CFTR actually does play a very key role in the development of pulmonary edema."

The study findings indicate that the loss of CFTR causes chloride to accumulate in the cells because it stops being transported out of them. The excess chloride triggers signaling that ends with an uncontrolled flow of calcium into the cells via a calcium channel. "The increased calcium concentration then causes the vascular cells to contract-much like the effect that calcium has on muscle cells," explains Prof. Kuebler. "This results in gaps between the cells – which allows fluid to spill out of the blood vessels. Chloride channels are therefore crucial in maintaining the barrier function of the pulmonary vessels."

The research team then addressed another question: How could they attenuate or prevent the pneumonia-induced loss of chloride channels in the pulmonary vessels? To answer this, the researchers used a therapeutic agent that is classed as a CFTR modulator and currently used to treat cystic fibrosis. In cystic fibrosis patients, a genetic mutation prevents the CFTR chloride channel from working properly in the mucosal cells of the airways, resulting in very viscous mucus. "Ivacaftor is a drug that increases the chances of the chloride channel opening, which helps the mucus to flow through the airways," says Dr. Erfinanda. "We wanted to see if it would also have a positive effect on the cells in the lungs' blood vessels."

Ivacaftor did make the chloride channels more stable: it led to less degradation in the channels than that typically caused by the lung's inflammatory processes. Experiments on animal models showed the same effect: treatment with ivacaftor increased the probability of surviving severe pneumonia, reduced lung injury, and resulted in much milder symptoms and a much better general condition than without the drug. "We really weren't expecting it to work so well," says Prof. Kuebler. "We hope our findings will pave the way for clinical trials to test the efficacy of CFTR modulators in pneumonia patients. Suppose this promising, pathogen-independent therapy finds its way into clinical practice. In that case, it could benefit a huge number of patients and prevent pneumonia from becoming life-threatening – even in the case of unknown pathogens."

Prof. Kuebler and his team are now planning research projects to develop other potential therapies based on the CFTR signaling pathway. They will also research which patients have an elevated risk of developing ARDS, so they can provide them with preventive, personalized treatment.

Reference:

Lasti Erfinanda, Lin Zou, Birgitt Gutbier, Laura Kneller, Sarah Weidenfeld, Laura Michalick, Disi Lei, Katrin Reppe, Luiz Gustavo Teixeira Alves, Bill Schneider , Caihong Li, Diana Fatykhova, Paul Schneider, Wolfgang Liedtke, Eisei Sohara, Timothy J. Mitchell, Achim D. Gruber, Andreas Hocke, Stefan Hippenstiel, Norbert Suttorp SCIENCE TRANSLATIONAL MEDICINE 7 Dec 2022 DOI: 10.1126/scitranslmed.abg8577