Irisin promotes odontogenic differentiation and angiogenesis in dental pulp cells: Study
Irisin promotes odontogenic differentiation and mineralization as well as has the potential for angiogenesis, suggests a study published in the International Endodontic Journal.
A group of researchers from Korea conducted a study to determine whether irisin, a newly discovered myokine that links exercise-induced and metabolic homeostasis, is able to promote odontogenic differentiation and angiogenesis in human dental pulp cells (HDPCs).
Cell viability in the presence of irisin was measured. Real-time PCR and Western blot analysis were performed to evaluate the expression levels of irisin, odontogenic and angiogenic markers. The involvement of mitogen-activated protein kinase (MAPK) and the protein kinase B (Akt) signalling pathway was evaluated by Western blot. To evaluate mineralization nodule formation, alkaline phosphatase (ALP) staining and alizarin red S staining was performed. Scratch wound assays were performed to evaluate the effects of irisin on cell migration. The data were analysed using a one-way analysis of variance (ANOVA) followed by Tukey post hoc test and Student's t-test.
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