Novel bone substitute may promote healing after tooth extraction: Study

In vitro, oral mucosa-derived epithelial cells (OECs) collected from 4-day-old Wistar rats were seeded on hydroxyapatite or CO3Ap and evaluated for adhesion, proliferation, migration, apoptosis, and morphology. Fibroblasts (FBs) were also analyzed for their ability to express collagen. In vivo, the extraction of maxillary right first (M1) and second molars (M2) of 6-week-old male Wistar rats were performed, followed by insertion of hydroxyapatite or CO3Ap granules into the M1 and M2 sites. The oral mucosal healing process was then evaluated histochemically after 7 and 14 days.

The results of the study are:

In vitro, high collagen expression by FBs in the CO3Ap group was observed and the surface analysis showed the spreading of the FBs on the CO3Ap surface. However, the activity of OECs was suppressed on CO3Ap. Two weeks after CO3Ap implantation, soft tissue healing was observed, and recovery of the connective tissue was observed on the remaining CO3Ap.

Thus, their results suggest that the formation of soft tissues, including connective tissue, was promoted by CO3Ap in the extraction socket within a short period.

Reference:

Effect of carbonate apatite as a bone substitute on oral mucosal healing in a rat extraction socket: in vitro and in vivo analyses using carbonate apatite by Yuki Egashira, et al. published in the International Journal of Implant Dentistry.

https://journalimplantdent.springeropen.com/articles/10.1186/s40729-022-00408-4