Flow cytometry useful alternative to microscopy for differentiation of bronchoalveolar lavage fluid leukocytes: Study
Flow cytometry useful alternative to microscopy for differentiation of bronchoalveolar lavage fluid leukocytes suggests a study published in the Chest.
Microscopy is currently the gold standard to differentiate BAL fluid (BALF) leukocytes. However, local expertise for microscopic BALF leukocyte differentiation is often unavailable in clinical practice. A new automated flow cytometric method for BALF leukocyte differentiation, using four antibodies (anti-CD45, anti-CD66b, anti-HLA-DR, anti-CD52) given to human BALF in one tube, was developed and prospectively validated in 745 unselected, subsequent BALF samples from patients with interstitial lung diseases (455 patients), infectious diseases (196 patients), and other diseases (94 patients). Flow cytometry and traditional microscopy were performed by separate investigators in a double-blind fashion. Results were compared using Spearman`s correlation, Deming regression, and Bland-Altman analysis.
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