Functional rejuvenation of Dental pulp stem cells novel and promising therapeutic strategy for pulpitis

Written By : Dr. Shravani Dali |Medically Reviewed By : Dr. Kamal Kant Kohli Published On 2022-10-21T20:15:46+05:30 | Updated On 21 Oct 2022 8:15 PM IST

Dental pulp stem cells functional rejuvenation is novel and promising therapeutic strategy for pulpitis. CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation and help rejuvenate dental pulp stem cells, according to a new study.

The study has been published in the International Dental Journal

Dental pulp stem cells (DPSCs) are a type of mesenchymal stem cell possessing self-renewal and multilineage differentiation capabilities. The dysfunction of DPSCs is related to the pathologic process of pulpitis. The participation of circular RNAs (circRNAs) in DPSC differentiation has been identified. This work focussed on exploring the functions and mechanism of circFKBP5 in DPSC dysfunction evoked by lipopolysaccharide (LPS).

The viability and apoptosis of human DPSCs (hDPSCs) were determined using Cell Counting Kit-8 assay and flow cytometry. Inflammation was analysed by measuring the release of inflammatory cytokines. The osteogenic differentiation of hDPSCs was investigated by performing alkaline phosphatase (ALP) staining and alizarin red S staining and detecting the changes of ALP and runt-related transcription factor 2 (RUNX2) proteins. The dual-luciferase reporter, RNA immunoprecipitation (RIP), and pull-down assays were used to confirm the binding between miR-708-5p and circFKBP5 or G-protein-coupled receptor (GPCR)–kinase interacting protein 2 (GIT2).

Results:

CircFKBP5 expression was decreased in hDPSCs and, functionally, reexpression of circFKBP5 attenuated LPS-induced apoptosis, inflammation, and inhibition of proliferation ability and osteogenic differentiation in hDPSCs.
Mechanistically, circFKBP5 acted as a sponge for miR-708-5p, which was verified to target GIT2.
LPS induced miR-708-5p expression in hDPSCs, and knockdown of miR-708-5p protected against LPS-evoked hDPSC dysfunction.
Besides, GIT2 expression was decreased in hDPSCs after LPS treatment.
Rescue experiments showed that GIT2 could mediate the protective functions of circFKBP5 on hDPSCs under LPS treatment.

CircFKBP5 could protect against LPS-induced apoptosis, inflammation, and osteogenic differentiation inhibition in hDPSCs via the miR-708-5p/GIT2 axis.

Reference:

Cuiwei Liang, Wenmiao Li, Qian Huang, Qitao Wen. CircFKBP5 Suppresses Apoptosis and Inflammation and Promotes Osteogenic Differentiation, International Dental Journal, 2022, ISSN 0020-6539. https://doi.org/10.1016/j.identj.2022.08.001

International Dental JournalCircFKBP5ApoptosisInflammationosteogenic Differentiation

Source : International Dental Journal

Dr. Shravani Dali

Dr. Shravani Dali has completed her BDS from Pravara institute of medical sciences, loni. Following which she extensively worked in the healthcare sector for 2+ years. She has been actively involved in writing blogs in field of health and wellness. Currently she is pursuing her Masters of public health-health administration from Tata institute of social sciences. She can be contacted at editorial@medicaldialogues.in.

Dr. Kamal Kant Kohli

Dr Kamal Kant Kohli-MBBS, DTCD- a chest specialist with more than 30 years of practice and a flair for writing clinical articles, Dr Kamal Kant Kohli joined Medical Dialogues as a Chief Editor of Medical News. Besides writing articles, as an editor, he proofreads and verifies all the medical content published on Medical Dialogues including those coming from journals, studies,medical conferences,guidelines etc. Email: drkohli@medicaldialogues.in. Contact no. 011-43720751