Rapid test for influenza viruses: Simply taste your way to infection

Influenza is much more than just a nasty cold-it is one of the most dangerous infectious diseases worldwide, claiming around half a million lives every year.

What makes it particularly insidious is that flu viruses are contagious even before the first symptoms appear. Despite this threat, the diagnostic options available to date are far from ideal. They are often expensive, complicated to use and simply unavailable in many poorer regions of the world.

A research team led by Professor Lorenz Meinel from the University of Würzburg has developed the technology behind a new self-test for influenza, recently published in ACS Central Science.

Detecting Flu Infections Quickly and Easily

The new principle could make flu diagnosis quicker, cheaper and easier in the future. Anyone could use it anytime, anywhere, for example in the form of chewing gum or a lollipop that reacts to flu viruses in the saliva of infected people and releases a flavouring. In the mouths of non-infected individuals, however, nothing would happen. This would allow those affected to detect an infection within minutes – without the need for a laboratory, electricity or medical personnel.

‘This strategy opens up new possibilities for the early detection and control of influenza worldwide,’ says Lorenz Meinel, who heads the Chair of Drug Formulation and Delivery at the University of Würzburg.

Flavouring Plus Virus-specific Building Block

The new diagnostic tool consists of the sensor molecule thymol – a natural substance found in thyme, among other things – and a virus-specific sugar building block. When it comes into contact with active influenza viruses, these release the thymol, creating a clearly recognisable taste in the mouth.

‘Instead of relying on expensive and complicated testing procedures, we use the natural human sensory system – taste – as a tool for the early detection of infections,’ says Lorenz Meinel.

The principle is flexible: both the flavour carrier and the recognition component can be adapted. For example, the system can be equipped with sweet, bitter or salty flavours – even child-friendly ones. It can also be transferred to different pathogens.

‘For other infections, for example, the virus-specific sugar component could be replaced by a bacteria-specific peptide. The underlying functionality would remain the same,’ explains Lorenz Meinel. The method thus opens up new possibilities for low-threshold diagnostics of viral and bacterial infections – from influenza to future pathogens that are not yet known today.

Next Step: Developing Specific Applications

The researchers are now working on incorporating the sensors into chewing gum or lollipops and making the diagnostic system suitable for mass production. To this end, it is collaborating with FlareOn Biotech GmbH, a start-up founded in 2024 by the University of Würzburg. The development process is expected to take around four years.

The team is convinced that such applications are particularly suitable for critical locations such as schools, nurseries and retirement homes. They could be of crucial importance in containing outbreaks of infection, especially in poorer countries.

Long-term Goal: Tracking the Spread of Influenza in Real Time

The researchers are already thinking further ahead: As soon as an application is available, they would like to ask all users to voluntarily record positive influenza test results using a smartphone app. This data could provide a previously unattainable online overview of the situation, enabling the spread of influenza viruses to be tracked in real time.

Artificial intelligence could then also be used to predict where epidemics or pandemics might develop. This would enable the World Health Organisation, governments, local authorities and even individuals to take precautionary measures at an early stage.

Sponsors and Participants in the Project

The Federal Ministry of Research, Technology and Space provided financial support for the work as part of the joint project ‘Influ Kau’.

The following were involved:

Dr Heinrich Jehle from the start-up FlareOn Biotech GmbH in Frickenhausen (Esslingen, Baden-Württemberg) contributed to the financing and regulatory advice.

Professors Christian Linz (University Hospital Würzburg, now University Hospital Cologne) and Stephan Hackenberg (University Hospital Würzburg) as well as their teams collected the clinical samples and determined and documented the patients' state of health.

Professor Jürgen Seibel (Institute of Organic Chemistry, University of Würzburg) and his doctoral student Marcel Groß co-developed the synthesis of the sensor molecule.

Professor Carlos A. Guzmán (Head of the Department of Vaccinology and Applied Microbiology at the Helmholtz Centre for Infection Research in Braunschweig, HZI) conducted investigations on influenza virus particles with teams led by Dr Peggy Riese and Dr Stephanie Trittel.

Reference:

Martina Raschig, Marcus Gutmann, Josef Kehrein, Eberhard Heller, Michael Bomblies, Marcel Groß, A Viral Neuraminidase-Specific Sensor for Taste-Based Detection of Influenza, ACS Central Science, DOI: 10.1021/acscentsci.5c01179.