Poor oral hygiene may be a significant risk factor for metabolic syndrome

A total of 35 Japanese patients admitted to the Eguchi Hospital and Saga Medical School for the treatment of MS were enrolled for the study. MS was diagnosed based on waist circumference (as a measure of central obesity) ≥85 cm for men and ≥90 cm for women and/or a visceral fat area ≥100 cm². Patient body mass index was calculated as the body weight in kilograms divided by the square of the height in meters (kg/m²). An abdominal computed tomography (CT) with the unenhanced spiral acquisition was also performed to calculate intramuscular adipose tissue content (IMAC) in the lumbar muscles.

The following were the key findings of this study-

a. Anti‐Porphyromonas gingivalis (Pg) antibody titers positively correlated with intramuscular adipose tissue content (IMAC), fasting blood glucose, and HOMA‐IR in metabolic syndrome patients.

b. In C57BL/6J mice fed a high‐fat diet, recipients of oral Pg (HFPg) had impaired glucose tolerance, insulin resistance and higher IMAC compared to recipients of saline (HFco).

c. The soleus muscle in HFPg mice exhibited fat infiltration and lower glucose uptake with higher Tnfa expression and lower insulin signaling than in HFco mice.

d. Gene set enrichment analysis showed that TNFα signaling via NFκB gene set was enriched in the soleus muscle of HFPg mice.

e. Moreover, TNF‐α also decreased glucose uptake in C2C12 myoblast cells in vitro.

f. Based on 16S rRNA sequencing, Pg administration altered the gut microbiome, particularly by decreasing the abundance of the genus Turicibacter.

g. The microbial network of the gut microbiome was dramatically changed by Pg administration.

Hence, the authors concluded that "infection with Pg is a risk factor for metabolic syndrome and skeletal muscle metabolic dysfunction via gut microbiome alteration indicating that periodontal bacteria induce insulin resistance and decrease glucose uptake in skeletal muscle by altering the gut microbiota."