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Synbiotic-fluoride enhances enamel remineralization with no biocompatibility concerns
The synbiotic-fluoride (SF) therapy enhances enamel remineralization with no biocompatibility concerns suggests a new study published in the Journal of Dentistry
The objectives of the present study were to examine the – a) enamel remineralization potential of synbiotic-fluoride (SF) therapy using a multi-species bacterial pH-cycling model; and b) cytotoxic and genotoxic effects of SF therapy extracts.
The SF therapy group comprised of 2% arginine (Arg), 0.2% NaF, and a probiotic Lactobacillus rhamnosus GG (LRG). The intervention groups studied were: 1) No treatment; 2) 2% Arg; 3) 0.2% NaF; 4) LRG; 5) 2% Arg+0.2% NaF; 6) 2% Arg+LRG; 7) 0.2% NaF+LRG; and 8) 2% Arg+0.2% NaF+LRG (SF therapy). The enamel remineralization potential of SF therapy was investigated under cariogenic biofilm challenge; while the cytotoxicity and genotoxicity of SF therapy extracts were examined on HGF-1 and Chinese hamster fibroblast V79, respectively. To determine the remineralization effect, the specimens were subjected to mineral density (MD) assessment using micro-CT, Ca/P molar ratio with SEM-EDX, and enamel fluoride uptake (EFU) estimates. The HGF-1 proliferation assessment was quantified using MTT/CCK-8 assays with qualitative analysis by nuclei staining Hoechst-based fluorescence imaging. The genotoxicity was determined by micronuclei formation test.
Results
- Mineral gain and % remineralization derived from MD assessment for the SF therapy were significantly higher than the other groups (p<0.05).
- The % ΔCa/P for the SF and 2% Arg+0.2% NaF were significantly higher than the other groups (p<0.05).
- The SF and 2% Arg+0.2% NaF groups had the highest EFU compared to the other groups (p<0.05).
- No significant difference in the % viable HGF-1 cells were observed between the treatment interventions and no treatment group (p>0.05).
- Compared to the EMS-positive control, the micronuclei formation for all the intervention groups was significantly lower (p<0.05), with no significant difference among the treatment groups (p>0.05).
The SF therapy enhanced enamel remineralization with no biocompatibility concerns. With the enhanced enamel remineralization potential discerned in the present study, the SF therapy can be used as a promising caries-preventive agent targeted for high caries-risk individuals.
Reference:
Mohammed Nadeem BIJLE, Mohamed Mahmoud ABDALLA, Chun Hung CHU, Cynthia Kar Yung YIU. Synbiotic-fluoride synergism on enamel remineralization, cytotoxicity and genotoxicity, Journal of Dentistry, 2022, 104356, ISSN 0300-5712. https://doi.org/10.1016/j.jdent.2022.104356.
Keywords
Arginine, fluoride, remineralization, synbiotictoxicity, Journal of Dentistry, Mohammed Nadeem BIJLE, Mohamed Mahmoud ABDALLA, Chun Hung CHU, Cynthia Kar Yung YIU
Dr. Shravani Dali has completed her BDS from Pravara institute of medical sciences, loni. Following which she extensively worked in the healthcare sector for 2+ years. She has been actively involved in writing blogs in field of health and wellness. Currently she is pursuing her Masters of public health-health administration from Tata institute of social sciences. She can be contacted at editorial@medicaldialogues.in.
Dr Kamal Kant Kohli-MBBS, DTCD- a chest specialist with more than 30 years of practice and a flair for writing clinical articles, Dr Kamal Kant Kohli joined Medical Dialogues as a Chief Editor of Medical News. Besides writing articles, as an editor, he proofreads and verifies all the medical content published on Medical Dialogues including those coming from journals, studies,medical conferences,guidelines etc. Email: drkohli@medicaldialogues.in. Contact no. 011-43720751